PLoS Pathog 17(12):Д®ditor: Donna Neumann, University of Wisconsin-Madison, UNITED STATES (2021) The RGD-binding integrins О±vОІ6 and О±vОІ8 are receptors for mouse adenovirus-1 and -3 infection. These integrins are highly conserved in other mammals, and may favour cross-species virus transmission.Д¬itation: Bieri M, Hendrickx R, Bauer M, Yu B, Jetzer T, Dreier B, et al. Together, the RGDLXXL-bearing FKs are part of an essential mechanism for M1/M3 infection engaging murine and human О±vОІ6/8 integrins. Molecular docking simulations of ternary complexes revealed a bent conformation of RGDLXXL-containing FK-M3 peptides on the subunit interface of О±vОІ6/ОІ8, where the distal leucine residue dips into a hydrophobic pocket of ОІ6/8, the arginine residue ionically engages О±v aspartate215, and the aspartate residue coordinates a divalent cation in О±vОІ6/ОІ8. Soluble integrin О±vОІ6, and synthetic peptides containing the RGDLXXL sequence derived from FK-M1, FK-M3 and foot and mouth disease virus coat protein strongly interfered with M1/M3 infections, in agreement with high affinity interactions of FK-M1/FK-M3 with О±vОІ6/О±vОІ8, determined by surface plasmon resonance measurements.

Anti-integrin antibodies potently blocked M1 and M3 binding and infection of CMT-93 cells and hО±vОІ8-positive M000216 cells. Ectopic expression of mОІ6 in B16 cells strongly enhanced M1 and M3 binding, infection, and progeny production comparable with mО±vОІ6-positive CMT-93 cells, whereas mОІ8 expressing cells were more permissive to M1 than M3. Profiling of murine and human cells expressing RGD-binding integrins suggested that О±vОІ6 and О±vОІ8 heterodimers are associated with M1 and M3 infections. Recombinant M1 or M3 FKs cross-blocked M1 and M3 but not M2 infections. To identify the receptors mediating MAdV infection we generated five novel reporter viruses for MAdV-1/-2/-3 (M1, M2, M3) transducing permissive murine (m) CMT-93 cells, but not B16 mouse melanoma cells expressing mCAR, human (h) CD46 or hDSG-2. While most HAdVs initiate infection by high affinity/avidity binding of their fiber knob (FK) protein to either coxsackievirus AdV receptor (CAR), CD46 or desmoglein (DSG)-2, MAdV-1 (M1) infection requires arginine-glycine-aspartate (RGD) binding integrins. Special thanks go to all those who have contributed and offered refinements, including the TFT Foundation Research Committee-Bob Bray, Jim McAninch, Bob Pasahow, and Rita Weinberg.Mammalian adenoviruses (AdVs) comprise more than ~350 types including over 100 human (HAdVs) and just three mouse AdVs (MAdVs). Robson, Roger Ludwig, Celestin Mitabu and Caitlin Phillips. Effectiveness of Thought Field Therapy Provided by Newly Instructed Community Workers to a Traumatized Population in Uganda: A Randomized Trial.Recent evidence for effectiveness of TFT for treating PTSD.YouTube Presentations on Thought Field Therapy В®.Articles, Books, and DVDs on Diet and Toxins.CDs and DVDs Related to Thought Field Therapy В®.CDs and DVDs on Offshoots of Thought Field Therapy В®.CDs and DVDs in Which Thought Field Therapy В® is Mentioned.CDs and DVDs on Thought Field Therapy В®.Books on Topics Related to Thought Field Therapy В®.Books on Offshoots of Thought Field Therapy В®.Books in Which Thought Field Therapy В® is Mentioned.Articles on Topics Related to Thought Field Therapy В®.Articles on Offshoots of Thought Field Therapy В®.Articles on Heart Rate Variability with Applications to Thought Field Therapy В®.Articles in Which Thought Field Therapy В® is Mentioned.Articles on Thought Field Therapy В® from the Japanese TFT Journal.Research on Offshoots of Thought Field Therapy В®.

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